GeneTex
  • Country / Location Selection

United States (US)

WISP1 antibody

Anti-WISP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04633
Anti-WISP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04633
Anti-WISP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX04633
Anti-WISP1 antibody used in Western Blot (WB). GTX04633
Anti-WISP1 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX04633
Anti-WISP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04633

Cat. No. GTX04633

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr, IHC-P (cell pellet)

Reactivity

Human, Mouse, Rat
Package
100 μg ($469)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5 μg/mL
IHC-P 0.5-1 μg/mL
IHC-Fr 0.5-1 μg/mL
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
By heat

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 mg Na₂HPO₄, 0.9 mg NaCl, 5 mg BSA

Preservative

0.05 mg Thimerosal, 0.05 mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1, different from the related mouse and rat sequences by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cellular communication network factor 4 , WISP1 , WISP1-OT1 , WISP1-UT1 , WISP1c , WISP1i , WISP1tc

Cellular Localization

Secreted

Background

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-WISP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04633

GTX04633 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04633 WISP1 antibody.

Anti-WISP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04633

GTX04633 IHC-P Image

IHC-P analysis of rat pancreas tissue using GTX04633 WISP1 antibody.

Anti-WISP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX04633

GTX04633 IHC-Fr Image

IHC-Fr analysis of rat cardiac muscle tissue using GTX04633 WISP1 antibody.
Dilution : 1 μg/mL

Anti-WISP1 antibody used in Western Blot (WB). GTX04633

GTX04633 WB Image

WB analysis of various samples using GTX04633 WISP1 antibody.
Lane 1 : Rat heart tissue lysate (50 μg)
Lane 2 : Rat kidney tissue lysate (50 μg)
Lane 3 : Rat lung tissue lysate (50 μg)
Lane 4 : A549 whole cell lysate (40 μg)
Lane 5 : A431 whole cell lysate (40 μg)
Lane 6 : COLO320 whole cell lysate (40 μg)
Dilution : 0.5 μg/mL

Anti-WISP1 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX04633

GTX04633 IHC-P (cell pellet) Image

IHC-P (cell pellet) analysis of MCF-7 cells using GTX04633 WISP1 antibody.

Anti-WISP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04633

GTX04633 IHC-P Image

IHC-P analysis of human placenta tissue using GTX04633 WISP1 antibody.

REFERENCE

There are currently no references for WISP1 antibody (GTX04633). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for WISP1 antibody (GTX04633). Be the first to share your experience with this product.
Package List Price ($)
$ 469